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yloB [2017-09-28 13:04:09]
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yloB [2017-09-28 13:04:09]

similar to the E. coli MgtA magnesium transporter
locus
BSU15650
pI
8.71
mw
97.10 kDa
protein length
890 aa Sequence Blast
gene length
2670 bp Sequence Blast
function
unknown
product
unknown
essential
no
ec
3.6.3.8
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.4|Metal ion homeostasis/ Other]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,637,965 → 1,640,637

    The protein

    Catalyzed reaction/ biological activity

  • ATP + H2O + Ca2+(Cis) = ADP + phosphate + Ca2+(Trans) (according to Swiss-Prot)
  • there is no indication for an implication of [protein|7821A09DADC6EAF589A882F05CC34BE900E06F2A|YloB] in Mg2+ uptake (based on mutant phenotypes, failure to complement mutants defective in Mg2+ uptake) [Pubmed|24415722]

    • Protein family

  • Type IIA subfamily (according to Swiss-Prot)

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|7821A09DADC6EAF589A882F05CC34BE900E06F2A|yloB]-[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]

    description

  • [pubmed|22383849]

    • regulation

  • expressed during sporulation in the mother cell [pubmed|12161109]
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    Biological materials

    Mutant

  • MGNA-B139 (yloB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1138 NBRP B. subtilis, Japan]
  • BKE15650 (Δ[gene|7821A09DADC6EAF589A882F05CC34BE900E06F2A|yloB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCGCTCGTCCACTCCC, downstream forward: _UP4_TAAATTCATATGATATAATC
  • BKK15650 (Δ[gene|7821A09DADC6EAF589A882F05CC34BE900E06F2A|yloB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCGCTCGTCCACTCCC, downstream forward: _UP4_TAAATTCATATGATATAATC

    • References

  • 24415722